SIRT5 is associated with asthenozoospermia and regulates GC‑2 spd cell proliferation and apoptosis
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- Published online on: July 23, 2025 https://doi.org/10.3892/mmr.2025.13634
- Article Number: 269
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Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
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Abstract
Infertility is a multifactorial condition that affects ~7% of the male population. The mechanism of male infertility primarily involves intrinsic and environmental factors, of which genetic defects are key for its occurrence. Sirtuin 5 (SIRT5) is primarily localized in mitochondria and associated with male reproduction. SIRT5 is downregulated in asthenozoospermic sperm compared with normal sperm. To explore the role of SIRT5 in male reproduction, Cell Counting Kit‑8, EdU, wound healing, and apoptosis assayswere performed in GC‑2 spd cells (mouse spermatocytes) which revealed that SIRT5 inhibited apoptosis and promoted cell proliferation and migration. Western blotting was performed and the results showed that SIRT5 was involved in the proliferation of GC‑2 spd cells by regulating the PI3K/AKT signaling pathway. The present study demonstrated a mechanism of male infertility, which may aid in its treatment.
