Brevilin A inhibits IL‑17A‑induced inflammation in psoriasis by modulating HSP 70&nbsp;

Li,Chia-Ling; Yeh,Kuei-Ying; Huang,Wen-Nan; Yen,Chung-Yang; Wang,Kai-Chun; Liao,En-Chih; Chou,Ting-Yu; Huang,Hung-Sen; Yu,Sheng-Jie

doi:10.3892/mmr.2025.13521

Brevilin A inhibits IL‑17A‑induced inflammation in psoriasis by modulating HSP 70

Authors:
- Chia-Ling Li
- Kuei-Ying Yeh
- Wen-Nan Huang
- Chung-Yang Yen
- Kai-Chun Wang
- En-Chih Liao
- Ting-Yu Chou
- Hung-Sen Huang
- Sheng-Jie Yu
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Affiliations: Children's Medical Center, Taichung Veterans General Hospital, Taichung 407, Taiwan, R.O.C., Department of Physical Therapy, College of Medical and Health Care, Hungkuang University, Taichung 433, Taiwan, R.O.C., Division of Allergy, Immunology, and Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407, Taiwan, R.O.C., School of Medicine, National Yang Ming Chiao Tung University, Taipei 112, Taiwan, R.O.C., Division of Allergy‑Immunology-Rheumatology, Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, R.O.C., Institute of Biomedical Sciences, MacKay Medical College, New Taipei City 252, Taiwan, R.O.C., Department of Medical Research, Taichung Veterans General Hospital, Taichung 407, Taiwan, R.O.C., Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, R.O.C., Integrated Care Center of Psoriatic Disease, Taichung Veterans General Hospital, Taichung 407, Taiwan, R.O.C.
Published online on: April 8, 2025 https://doi.org/10.3892/mmr.2025.13521
Article Number: 156
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Psoriasis, which is characterized by keratinocyte hyperproliferation, presents complex management challenges. The heat shock protein 70 (HSP 70) family, which is essential in protein folding and stress responses, also modulates inflammation, suggesting its therapeutic potential in inflammation‑driven diseases. The present study aimed to explore the effects of brevilin A, a natural compound known to alleviate imiquimod‑induced psoriasis, on HSP 70 expression and proinflammatory cytokine production in HaCaT cells stimulated with IL‑17A. An HSP 70 inhibitor was used to determine its role in cytokine regulation, and the effect of brevilin A on skin pathology in mice was examined via immunohistochemistry and hematoxylin and eosin staining. The results revealed that brevilin A markedly decreased IL‑6 and IL‑8 levels after IL‑17A stimulation at both 9 and 24 h in HaCaT cells, and increased HSP 70 and HSP 90 expression levels. Notably, the brevilin A‑induced suppression of cytokine levels was reversed when cells were co‑treated with the HSP 70 inhibitor. In vivo, brevilin A enhanced HSP 70 expression and reduced skin hyperproliferation. These findings suggested that brevilin A may modulate HSP 70 expression and dampen the inflammatory response induced by IL‑17A, indicating its potential as an innovative treatment for psoriasis.

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